RockHound
02-13-2008, 09:50 PM
http://i270.photobucket.com/albums/jj85/placer_mines/anammoxboifiltercopy-1.jpg
Well, I am going to build one.
Or rather, a combination of 3 type filters.
(just to see if it will function)
Certainly not your regular type filter system.
Combination of a noxic degrading to anoxic slow flow bio-filter.
Combined with a high flow trickle bio-filter tower.
Combined with a shower type bio-filter combination degassing tower.
Overall combined flow rate is projected @ 600 GPH.
It is NOT as complex as it appears.
PVC pipe & valves, food grade plastic drums & assorted bio-housing-media.
If you are curious about the principles behind this puppy?
Denitrification is a dissimilatory microbial redox process where nitrogen oxides (NO3−, NO2−) are reduced stepwise to gaseous end products (NO, N2O, N2) which are then gassed off in an agitated or aerated air/water interface.
In the process of denitrification, nitrate, the form of nitrogen that results from the completion of the nitrification process, is converted to nitrogen gas, utilizing facultative heterotrophic bacteria. The process of denitrification occurs under anoxic conditions as follows:
NO3 + Denitrifying Bacteria + Organic Carbon Nitrogen Gas + Water + Alkalinity
There are two steps required to achieve denitrification, i.e. nitrate removal.
Step 1- The nitrification process is reversed, and nitrate (NO3) is converted back to nitrite (NO2).
Step 2- Nitrite is converted to nitric oxide (NO), then nitrous oxide (N2O) and finally to nitrogen gas (N2).
The completion of the denitrification process can be summarized as follows:
NO3 > NO2 > NO > N2O > N2
There are two main requirements for successful denitrification.
1. Anoxic environment
Since facultative heterotrophs prefer to respire using DO, the denitrifiers will continue to respire aerobically as long as DO is available.
It is only when the DO is depleted that denitrifiers begin using nitrate for respiration, which begins the denitrification reaction.
2. Sufficient amount of organic carbon
Without organic carbon as the food supply, facultative heterotrophs cannot continue to grow,multiply and thrive. Organic carbon can be supplied to the denitrification phase of biological treatment using influent water (which contains cBOD), and/or supplemented with either an ethanol, or a sugar solution.
One final point: Biological denitrification converts nitrate to nitrogen gas. Which is typically gassed off in an aerated chamber that follows the denitrification process.
ANAMMOX = http://www.biochemsoctrans.org/bst/034/0174/0340174.pdf
Also google "CANON reactor" and/or "coil denitrifier".
~~~~~~~~~
Also see:
Allison SM, Prosser JI 1993: Ammonia oxidation at low pH by attached populations of nitrifying bacteria. Soil Biol. Biochem., 25, 935–941.
•
Altmann D, Stief P, Amann R, de Beer D, Schramm A 2003: In situ distribution and activity of nitrifying bacteria in freshwater sediment. Environ. Microbiol., 5, 798–803.
•
Arp DJ, Stein LY 2003: Metabolism of inorganic N compounds by ammonia-oxidizing bacteria. Crit. Rev. Biochem. Mol. Biol., 38, 471–495.
•
Avrahami S, Liesack W, Conrad R 2003: Effects of temperature and fertilizer on activity and community structure of soil ammonia oxidizers. Environ. Microbiol., 5, 691–705.
•
Bartosch S, Hartwig C, Spieck E, Bock E 2002: Immunological detection of Nitrospira-like bacteria in various soils. Microb. Ecol., 43, 26–33.
•
Bateman EJ, Baggs EM 2005: Contributions of nitrification and denitrification to N2O emissions from soils at different water-filled pore space. Biol. Fertil. Soil, 41, 379–388.
•
Beaumont HJ, Hommes NG, Sayavedra-Soto LA, et al. 2002: Nitrite reductase of Nitrosomonas europaea is not essential for production of gaseous nitrogen oxides and confers’ tolerance to nitrite. J. Bacteriol., 184, 2557–2560.
•
Beman JM, Francis CA 2006: Diversity of ammonia-oxidizing archaea and bacteria in the sediments of a hypernutrified subtropical estuary: Bahia del Tobari, Mexico. Appl. Environ. Microbiol., 72, 7767–7777.
•
Bintrim SB, Donohue TJ, Handelsman J, Roberts GP, Goodman RM 1997: Molecular phylogeny of Archaea from soil. Proc. Natl Acad. Sci. USA, 94, 277–282.
•
Bowatte S, Jia Z, Ishihara R, Asakawa S, Kimura M 2006a: Characterization of ammonia-oxidizing bacteria associated with weeds in a Japanese paddy field using amoA gene fragments. Soil Sci. Plant Nutr., 52, 593–600.
•
Bowatte S, Jia Z, Ishihara R, Nakajima Y, Asakawa S, Kimura M 2006b: Molecular analysis of the ammonia-oxidizing bacterial community in the surface soil layer of Japanese paddy field. Soil Sci. Plant Nutr., 52, 427–431.
•
Brierley EDR, Wood M 2001: Heterotrophic nitrification in an acid forest soil: isolation and characterisation of a nitrifying bacterium. Soil Biol. Biochem., 33, 1403–1409.
•
Brierley EDR, Wood M, Shaw PJA 2001: Influence of tree species and ground vegetation on nitrification in an acid forest soil. Plant and Soil, 229, 97–104.
•
Broda E 1977: Two kinds of lithotrophs missing in nature. Z. Allg. Mikrobiol., 17, 491–493.
•
Bruns MA, Stephen JR, Kowalchuk GA, Prosser JI, Paul EA 1999: Comparative diversity of ammonia oxidizer 16S rRNA gene sequences in native, tilled, and successional soils. Appl. Environ. Microbiol., 65, 2994–3000.
•
Buckley DH, Schmidt TM 2003: Diversity and dynamics of microbial communities in soils from agro-ecosystems. Environ. Microbiol., 5, 441–452.
•
Cabello P, Roldan MD, Moreno-Vivian C 2004: Nitrate reduction and the nitrogen cycle in archaea. Microbiology, 150, 3527–3546.
•
Castaldi S, Smith KA 1998: Effect of cycloheximide on N2O and production in a forest and an agricultural soil. Biol. Fertil Soils 27, 27–34.
•
Chain P, Lamerdin J, Larimer F et al. 2003: Complete genome sequence of the ammonia-oxidizing bacterium and obligate chemolithoautotroph Nitrosomonas europaea. J. Bacteriol., 185, 2759–2773.
•
Cho CM, Yan T, Liu X, Wu L, Zhou J, Stein LY 2006: Transcriptome of a Nitrosomonas europaea mutant with a disrupted nitrite reductase gene (nirK). Appl. Environ. Microbiol., 72, 4450–4454.
•
Chu H, Fujii T, Morimoto S et al. 2006: Community structure of ammonia-oxidizing bacteria under long-term application of mineral fertilizer and organic manure in a sandy loam soil. Appl. Environ. Microbiol., 73, 485–491.
•
Conrad R 1996: Soil microorganisms as controllers of atmospheric trace gases (H2, CO, CH4, OCS, N2O, and NO). Microbiol. Rev. 60, 609–640.
•
Crossman LC, Moir JW, Enticknap JJ, Richardson DJ, Spiro S 1997: Heterologous expression of heterotrophic nitrification genes. Microbiology 143, 3775–3783.
•
Daims H, Nielsen PH, Nielsen JL, Juretschko S, Wagner M 2000: Novel Nitrospira-like bacteria as dominant nitrite-oxidizers in biofilms from wastewater treatment plants: diversity and in situ physiology. Water Sci. Technol., 41, 85–90.
•
Dalsgaard T, Canfield DE, Petersen J, Thamdrup B, Acuna-Gonzalez J 2003: N2 production by the anammox reaction in the anoxic water column of Golfo Dulce, Costa Rica. Nature, 422, 606–608.
•
Daum M, Zimmer W, Papen H, Kloos K, Nawrath K, Bothe H 1998: Physiological and molecular biological characterization of ammonia oxidation of the heterotrophic nitrifier Pseudomonas putida. Curr. Microbiol., 37, 281–288.
•
De Boer W, Kowalchuk GA 2001: Nitrification in acid soils: micro-organisms and mechanisms. Soil Biol. Biochem., 33, 853–866.
•
Focht DD, Verstraete W 1977: Biochemical ecology of nitrification and denitrification. In Advances in Microbial Ecology, Vol. 1. Ed. M Alexander, pp. 135–214. Plenum Press,
New York
•
Francis CA, Roberts KJ, Beman JM, Santoro AE, Oakley BB 2005: Ubiquity and diversity of ammonia-oxidizing archaea in water columns and sediments of the ocean. Proc. Natl Acad. Sci. USA, 102, 14683–14688.
•
Freitag TE, Chang L, Clegg CD, Prosser JI 2005: Influence of inorganic nitrogen management regime on the diversity of nitrite-oxidizing bacteria in agricultural grassland soils. Appl. Environ. Microbiol., 71, 8323–8334.
•
Hastings RC, Ceccherini MT, Miclaus N, Saunders JR, Bazzicalupo M, McCarthy AJ 1997: Direct molecular biological analysis of ammonia-oxidizing bacteria population in cultivated soil plots treated with swine manure. FEMS Microbiol. Ecol., 23, 45–54.
•
Hayatsu M 1993: The lowest limit of pH for nitrification in tea soil and isolation of an acidophilic ammonia-oxidizing bacterium. Soil Sci. Plant Nutr., 39, 219–226.
•
Head IM, Hiorns WD, Embley TM, McCarthy AJ, Saunders JR 1993: The phylogeny of autotrophic ammonia-oxidizing bacteria as determined by analysis of 16S ribosomal RNA gene sequences. J. Gen. Microbiol., 139, 1147–1153.
•
Hooper AB, Terry KR 1979: Hydroxylamine reductase of Nitrosomonas production of nitric oxide from hydroxylamine. Biochim. Biophys. Acta, 571, 12–20.
•
Ida T, Takahashi R, Satoh K, Kogure M, Tokuyama T 2006: Classification of chemoautotrophic ammonia-oxidizing bacteria using pyruvate kinase genes as high-resolution phylogenetic markers. Soil Sci. Plant Nutr., 52, 284–290.
•
Jetten MS, de Bruijn P, Kuenen JG 1997: Hydroxylamine metabolism in Pseudomonas PB16: involvement of a novel hydroxylamine oxidoreductase. Antonie Van Leeuwenhoek., 71, 69–74.
•
Joo HS, Hirai M, Shoda M 2005: Characteristics of ammonium removal by heterotrophic nitrification-aerobic denitrification by Alcaligenes faecalis no. 4. J. Biosci. Bioeng., 100, 184–191.
•
Killham K 1986: Heterotrophic nitrification. In Nitrification: Special Publications of the Society for General Microbiology, Vol. 20. Ed. JI Prosser, pp. 117–126. IRL Press,
Oxford
•
Kimura M 2000: Anaerobic microbiology in waterlogged rice fields. In Soil Biochemistry. Eds J Bollag and G Stotzky, pp. 35–138. Marcel Dekker,
New York
•
Klotz MG, Arp DJ, Chain PS et al. 2006: Complete genome sequence of the marine, chemolithoautotrophic, ammonia-oxidizing bacterium Nitrosococcus oceani ATCC 19707. Appl. Environ. Microbiol., 72, 6299–6315.
•
Konneke M, Bernhard AE, de la Torre JR, Walker CB, Waterbury JB, Stahl DA 2005: Isolation of an autotrophic ammonia-oxidizing marine archaeon. Nature, 437, 543–546.
•
Kowalchuk GA, Stephen JR 2001: Ammonia-oxidizing bacteria: a model for molecular microbiology ecology. Annu. Rev. Microbiol. 55, 485–529.
•
Kuypers MM, Sliekers AO, Lavik G et al. 2003: Anaerobic ammonium oxidation by anammox bacteria in the Black Sea. Nature, 422, 608–611.
•
Laverman AM, Speksnijder AG, Braster M, Kowalchuk GA, Verhoef HA, Van Verseveld HW 2001: Spatiotemporal stability of an ammonia-oxidizing community in a nitrogen-saturated forest soil. Microb. Ecol., 42, 35–45.
•
Leininger S, Urich T, Schloter M et al. 2006: Archaea predominate among ammonia-oxidizing prokaryotes in soils. Nature, 422, 806–809.
•
MacLain JET, Martens DA 2006: N2O production by heterotrophic N transformations in a semiarid soil. Appl. Soil Ecol., 32, 253–263.
•
Meyer RL, Risgaard-Petersen N, Aller DE 2005: Correlation between anammox activity and the microscale distribution of nitrite in a subtropical mangrove sediment. Appl. Environ. Microbiol., 71, 6142–6149.
•
Moir JWB, Crossman LC, Spiro S, Richardson DJ 1996a: The purification of ammonia monooxygenase from Paracoccus denitrificans. FEBS Lett., 387, 71–74.
•
Moir JWB, Wehrfritz JM, Spiro S, Richardson DJ 1996b: The biochemical characterisation of a novel non-haem iron hydroxylamine oxidase from Paracoccus denitrificans GB17. Biochem. J., 319, 823–827.
•
Mulder A, van de Graaf AA, Robertson LA, Kuenen JG. 1995: Anaerobic ammonium oxidation discovered in a denitrifying fluidized bed reactor. FEMS Microbiol. Ecol., 16, 177–184.
•
Murase J, Itoh K, Kano M, Kimura M 2003: Molecular analysis of β-proteobacterial ammonia oxidizer populations in surface layers of a submerged paddy soil microcosm. Soil Sci. Plant Nutr., 49, 909–913.
•
Murase J, Shimizu M, Hayashi M, Matsuya K, Kimura M 2005: Vertical changes in bacterial communities in percolating water of a Japanese paddy field as revealed by PCR-DGGE. Soil Sci. Plant Nutr., 51, 83–90
•
Nakahara K, Tanimoto T, Hatano K, Usuda K, Shoun H 1993: Cytochrome P-450 55A1 (P-450dNIR) acts as nitric oxide reductase employing NADH as the direct electron donor. J. Biol. Chem., 268, 8350–8355.
•
Nakayama N, Okabe A, Toyota K, Kimura M, Asakawa S 2006: Phylogenetic distribution of bacteria isolated from the floodwater of a Japanese paddy field. Soil Sci. Plant Nutr., 52, 305–312.
•
Nicolaisen MH, Risgaard-Petersen N, Revsbech NP, Reichardt W, Ramsing NB 2004: Nitrification-denitrification dynamics and community structure of ammonia-oxidizing bacteria in a high yield irrigated Philippine rice field. FEMS Microbiol. Ecol., 49, 359–369.
•
Nugroho RA, Roling WF, Laverman AM, Zoomer HR, Verhoef HA 2005: Presence of Nitrosospira cluster 2 bacteria corresponds to N transformation rates in nine acid Scots pine forest soils. FEMS Microbiol. Ecol., 53, 473–481.
•
Ochsenreiter T, Selezi D, Quaiser A, Bonch-Osmolovskaya L, Schleper C 2003: Diversity and abundance of Crenarchaeota in terrestrial habitats studied by 16S RNA surveys and real-time PCR. Environ. Microbiol., 5, 787–797.
•
Okada N, Nomura N, Nakajima-Kambe T, Uchiyama H 2005: Characterization of the aerobic denitrification in Mesorhizobium sp. strain NH-14 in comparison with that in related rhizobia. Microbes Environ., 20, 208–215.
•
Okano Y, Hristova KR, Leutenegger CM et al. 2004: Application of real-time PCR to study effects of ammonium on population size of ammonia-oxidizing bacteria in soil. Appl. Environ. Microbiol., 70, 1008–1016.
•
Oline DK, Schmidt SK, Grant MC 2006: Biogeography and landscape-scale diversity of the dominant Crenarchaeota of soil. Microb. Ecol., 52, 480–490.
•
Park HD, Wells GF, Bae H, Criddle CS, Francis CA 2006: Occurrence of ammonia-oxidizing archaea in wastewater treatment plant bioreactors. Appl. Environ. Microbiol., 72, 5643–5647.
•
Patureau D, Zumstein E, Delgenes JP, Moletta R 2000: Aerobic denitrifiers isolated from diverse natural and managed ecosystems. Microb. Ecol., 39, 145–152.
•
Penton CR, Devol AH, Tiedje JM 2006: Molecular evidence for the broad distribution of anaerobic ammonium-oxidizing bacteria in freshwater and marine sediments. Appl. Environ. Microbiol., 72, 6829–6832.
•
Philippot L 2002: Denitrifying genes in bacterial and archaeal genomes. Biochim. Biophys. Acta, 1577, 355–376.
•
Phillips CJ, Harris D, Dollhopf SL, Gross KL, Prosser JI, Paul EA 2000: Effects of agronomic treatments on structure and function of ammonia-oxidizing communities. Appl. Environ. Microbiol., 66, 5410–5418.
•
Poth M, Focht D 1985: 15N kinetic analysis of production by Nitrosomonas europaea: an examination of nitrifier denitrification. Appl. Environ. Microbiol., 49, 1134–1141.
•
Prosser JI 1989: Autotrophic nitrification in bacteria. Adv. Microb. Physiol., 30,125–181.
•
Purkhold U, Wagner M, Timmermann G, Pommerening-Roser A, Koops HP 2003:16S rRNA and amoA-based phylogeny of 12 novel beta-proteobacterial ammonia-oxidizing isolates: extension of the dataset and proposal of a new lineage within the nitrosomonads. Int. J. Syst. Evol. Microbiol., 53, 1485–1494.
•
Sakai K, Nisijima H, Ikenaga Y, Wakayama M, Moriguchi M 2000: Purification and characterization of nitrite-oxidizing enzyme from heterotrophic Bacillus badius 1–73, with special concern to catalase. Biosci Biotechnol Biochem., 64, 2727–2730.
•
Schmid MC, Maas B, Dapena A et al. 2005: Biomarkers for in situ detection of anaerobic ammonium-oxidizing (anammox) bacteria. Appl. Environ. Microbiol., 71, 1677–1684.
•
Schmid MC, Risgaard-Petersen N, van de Vossenberg J et al. 2007: Anaerobic ammonium-oxidizing bacteria in marine environments: widespread occurrence but low diversity. Environ. Microbiol., 9,1476–1484.
•
Schmidt I, Bock E 1997: Anaerobic ammonia oxidation with nitrogen dioxide by Nitrosomonas eutropha. Arch. Microbiol., 167, 106–111.
•
Schmidt I, van Spanning RJ, Jetten MS 2004: Denitrification and ammonia oxidation by Nitrosomonas europaea wild-type, and NirK- and NorB-deficient mutants. Microbiology, 150, 4107–4114.
•
Schramm A, de Beer D, van den Heuvel JC, Ottengraf S, Amann R 1999: Microscale distribution of populations and activities of Nitrosospira and Nitrospira spp. along a macroscale gradient in a nitrifying bioreactor: quantification by in situ hybridization and the use of microsensors. Appl. Environ. Microbiol., 65, 3690–3696.
•
Schubert CJ, Durisch-Kaiser E, Wehrli B, Thamdrup B, Lam P, Kuypers MM 2006. Anaerobic ammonium oxidation in a tropical freshwater system (Lake Tanganyika). Environ Microbiol., 8, 1857–1863.
•
Shaw LJ, Nicol GW, Smith Z, Fear J, Prosser JI, Baggs EM 2006: Nitrosospira spp. can produce nitrous oxide via a nitrifier denitrification pathway. Environ. Microbiol., 8, 214–222.
•
Shiro Y, Fujii M, Iizuka T et al. 1995: Spectroscopic and kinetic studies on reaction of cytochrome P450nor with nitric oxide: implication for its nitric oxide reduction mechanism. J. Biol. Chem., 270, 1617–1623.
•
Simon J 2002: Enzymology and bioenergetics of respiratory nitrite ammonification. FEMS Microbiol. Rev., 26, 285–309.
•
Skiba U, Smith KA 2000: The control of nitrous oxide emissions from agricultural and natural soils. Glob. Change Sci., 2, 379–386.
•
Starkenburg SR, Chain PS, Sayavedra-Soto LA et al. 2006: Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium Nitrobacter winogradskyi Nb-255. Appl. Environ. Microbiol., 72, 2050–2063.
•
Stephen JR, McCaig AE, Smith Z et al. 1996: Molecular diversity of soil and marine 16S rRNA gene sequences related to beta-subgroup ammonia-oxidizing bacteria. Appl. Environ. Microbiol., 62, 4147–4154.
•
Strous M, Fuerst JA, Kramer EH et al. 1999: Missing lithotroph identified as new planctomycete. Nature, 400, 446–449.
•
Strous M, Pelletier E, Mangenot S et al. 2006: Deciphering the evolution and metabolism of an anammox bacterium from a community genome. Nature, 440, 790–794.
•
Su F, Takaya N, Shoun H 2004: Nitrous oxide-forming codenitrification catalyzed by cytochrome P450nor. Biosci. Biotechnol. Biochem., 68, 473–475.
•
Sugano A, Tsuchimoto H, Tun CC, Asakawa S, Kimura M 2005: Succession and phylogenetic profile of eubacterial communities in rice straw incorporated into a rice field: estimation by PCR-DGGE analysis. Soil Sci. Plant Nutr., 51, 51–60.
•
Sutka RL, Ostrom NE, Ostrom PH et al. 2006: Distinguishing nitrous oxide production from nitrification and denitrification on the basis of isotopomer abundances. Appl. Environ. Microbiol., 72, 638–644.
•
Tal Y, Watts JE, Schreier HJ 2005: Anaerobic ammonia-oxidizing bacteria and related activity in Baltimore inner harbor sediment. Appl. Environ. Microbiol., 71, 1816–1821.
•
Teske A, Alm E, Regan JM, Toze S, Rittmann BE, Stahl DA 1994: Evolutionary relationships among ammonia- and nitrite-oxidizing bacteria. J. Bacteriol., 176, 6623–6630.
•
Tiedje JM 1988: Ecology of denitrification and dissimilatory nitrate reduction to ammonium. In Biology of Anaerobic Microorganisms. Ed. AJB Zehnder, pp. 179–244. John Wiley & Sons, New York
•
Toh SK, Webb RI, Ashbolt NJ 2002: Enrichment of autotrophic anaerobic ammonium-oxidizing consortia from various wastewaters. Microb. Ecol., 43, 154–167.
•
Treusch AH, Leininger S, Kletzin A, Schuster SC, Klenk HP, Schleper C 2005: Novel genes for nitrite reductase and Amo-related proteins indicate a role of uncultivated mesophilic crenarchaeota in nitrogen cycling. Environ. Microbiol., 7, 1985–1995.
•
Van de Graaf AA, Mulder A, de Bruijn P, Jetten MS, Robertson LA, Kuenen JG 1995: Anaerobic oxidation of ammonium is a biologically mediated process. Appl. Environ. Microbiol., 61, 1246–1251.
•
Walker N, Wickramasinghe KN 1979: Nitrification and autotrophic nitrifying bacteria in acid tea soils. Soil Biol. Biochem., 11, 231–236.
•
Webster G, Embley TM, Prosser JI 2002: Grassland management regimens reduce small-scale heterogeneity and species diversity of β-proteobacterial ammonia oxidizer populations. Appl. Environ. Microbiol., 68, 20–30.
•
Wrage N, Velthof GL, Laanbroek HJ, Oenema O 2004: Nitrous oxide production in grassland soils: assessing the contribution of nitrifier denitrification. Soil Biol. Biochem., 36, 229–236.
•
Wrage N, Velthof GL, van Beusichem ML, Oenema O 2001: The role of nitrifier denitrification in the production of nitrous oxide. Soil Biol. Biochem., 33, 1723–1732.
•
Yokoyama K, Ohama T 2005: Effect of inorganic N composition of fertilizers on nitrous oxide emission associated with nitrification and denitrification. Soil Sci. Plant Nutr., 51, 967–972.
•
Zengler K, Toledo G, Rappe M et al. 2002: Cultivating the uncultured. Proc. Natl Acad. Sci. USA, 99, 15681–15686.
•
Zumft WG 1997: Cell biology and molecular basis of denitrification. Micobiol. Mol. Biol. Rev., 61, 533–616.
Powered by vBulletin® Version 4.2.2 Copyright © 2024 vBulletin Solutions, Inc. All rights reserved.