Originally Posted by
Dkarc@Aol.com
Sounds good Angel, but I would include more than 2 fish per tank. To make it a reliable/credible study you need atleast 6 replicates of each. So 6 fish per tank, 6 tanks of replicates (36 fish for softwater, 36 for hard water, and 36 for control). It sounds like a lot, but in fish nutrition/growth studies, replicates are extremely important as little things could effect a single replicate and could throw off the whole study if you dont have sufficient replicates to provide enough scientific information.
-Ryan