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brewmaster15
04-05-2002, 05:55 PM
Blackworm culture  (Lumbriculus variegatus) for use as a live fish food for the Hobbyist.
By Al Sabetta , 1/2002
 
This culture method is based on one that was kindly provided to me by Dr. Charles D. Drewes at Iowa State University for use in some of my science workshops. A variation of it is also recommended by Carolina Biological Supply Company for their cultures. ( The article is viewable at Carolina's website and was  written by Dr.Drewes)

To set up the culture, brown paper towels are placed in a suitable container such as a fish tank.  Clean water (De-ionized or Reverse osmosis water) is poured to a shallow depth of  several inch over that.  Black worms from a clean source are used to start the culture.  These are added to the culture. Aeration with an air stone is recommended.  This culture does not need to be refrigerated; it can be kept at room temperature. I have been keeping mine at 65-75 degrees F and they have been thriving.

The decomposing paper towels and associated micro flora/fauna will serve as food for the worms, additional food sources can also be added, such as flake fish food- use sparingly or it will foul the culture.  The worms will reproduce many by asexual means- breaking along their segmented bodies into 2 or more parts and regenerating into  individuals. They are capable of sexual reproduction but this is not usually the mode of reproduction in culture.  As the culture grows wastes will accumulate and the water should be changed weekly or more frequently as needed, add additional paper towels as needed.  

This is the basic culture design for blackworms used in science experiments and works very well. It has a few drawbacks for the aquarium hobbyist in that the paper towels start to breakdown pretty quick, and its tough to separate the worms from the mushy paper towels  to give you enough clean worms to feed your fish. It requires multiple washes. I have found that this can be helped by pouring some of the culture thru a mesh screen (window screen) first to remove the larger pieces of paper.  I have also  taken  a portion of the culture to be harvested and  loosely wrapped  it in  cheese cloth the day before needed. This is placed in a separate container of water.  The black Worms will make their way out of the cheese cloth and are easily collected in the clean water.

I have also started using heavier brown shopping bags in my cultures. I have found that they last much longer (up to a month as substrate), and are easier to separate the worms from than the brown paper towels.  By far the most promising substrate I have found yet is brown burlap, availible at hardware stores.  I am in week three of a trial (at the time of this writing) and the burlap is working great. The water is cleaner, the  burlap cloth holds up much better even though it is biodegradable. It requires additional food being added to the culture   since it breaks down slowly, but this is nutritionally better for the fish as you can control what the worms eat.   Burlap allows for more oxygen in the water because it is not a solid material like the paper towels and paper bags.  Finally it's extremely easy  to separate out the worms from this substrate.

Do not use chlorinated water, even the de-chlorinated city water is not a good to use because of all the processing it has gone thru. Purchase a gallon of Bottled  spring water if you have to. It will give you enough water to take care of a 10 Gallon fish tank culture for over a month.  Do not make the water too deep, especially if you do not add aeration. I am using 1-3 inches in my current cultures. Remember.. Black worms require oxygen or they will die!  Do not refrigerate this culture, they will not reproduce, refrigeration is good for storage of the live worms, but not culturing them. Do not use any processed, bleached papers as substrate.

Be sure to obtain you worms from a clean source. There are many organisms in the wild that eat these worms, including some leeches. You do not want these in your cultures.  

Some  sources for  Blackworms

1) Aquatic foods, 3133 N Argyle, Fresno, CA 93727
    3133 N. Argyle, Fresno, CA 93727
(559) 291-0623 -Info
(559) 291-0601 -Fax
blkworm@aol.com

2) Carolina Biological Supply
    2700 York Road
    Burlington, NC  27215-3398
 US Customers: 1- 800-334-5551  
  Canadian Customers 1-800-387-2474
  carolina@carolina.com

3) Berkshire Biological
    1-(800)-462-1382.
http://www.berkshirebio.com/index.html  

brewmaster15
04-05-2002, 05:55 PM
supplimental info....
Okay, heres the deal. I would set at least one of your cultures up with the brown paper towels 0r shopping bag. One layer with an inch of water (if you are using an airstione  you can use  several inches  of water.) This  is your backup culture , as its a proven  method. Though more difficult  to get the worms  from.  
  The Burlap  culture   I am trying is only about a month old, but is doing very fine. I have a single layer of burlap  in a  10gal tank . Spread the burlap out flat. I am aerating it with a 6 inch air stone, but any airstone should work.   Initially don't feed anything for a about a week ,  remember there will be biological  filtration isssues  here  just as  with a tank. Worms also create waste- I haven't tried it but I toyed with using Mulm as a food source as it would also give the culture nitro bacteria.   After a few days -week, you can feed a  small pinch of  flake- I use a meat lovers flake , mix it in the water.  I do this weekly, and if I see it hasn't been eaten in a few days  I change the water. You'll lose some  worms  with the dead  worms and debris when you pour it off. YOu can minimize this  by filling the tub with water stirring and letting settle a minute the live worms generally sink, then pour off.  
 At no time  should the culture smell foul. If it does then  there too much food, not enough air  or  bad bacteria is a probelm. __X_  says he uses an antibiotic in his tubiflex culture- I haven't used any but it may save a  culture if theres  a probelm. The key to the getting any of these cultures started is to use healthy starter material. When  you get  your blackworms they'll be on ice  probably.   Rinse them in Ice cold water until the water is clear.  Now put them in a tub, with  just barely enough water  to cover them  and let them come to room   temperature. Once they do you'llsee  them actively moving about. Now wait an hour or so, check them periodically. You'll notice the really healthy one clump up. Also you'll notice that they'll actually try  to climb out  of the  containers at thecorners.It looks like a sci-fi movie . These are your healthiest worms and   are good for culture.  Watch out for leeches, and worms that are clear (dead)
Hope this helped Harry

Additional info....
  Cultures take time to establish. Only use a handful of healthy worms to get the culture started, Too many and  you'll foul the water. There numbers will grow naturally, as the burlap  breaks down.   Oxygen is key here ,as well as monitoring it carefully in the beginning. If you can keep several cultures going, and  try different parameters, experiment on the water depth  and density of worms, and amount of  aeration. Good luck, let me know how you make out. I'll be trying a new substrate  this week end in a culture - agricultural grade vermiculite enriched with mulm,I'll let you know how it goes !


supplimental 2....
Just an update, the paper  method works , the burlap works  , but  you need to really aerate  it, and change water  alot. I have   not been able to get  huge amounts of worms  though. For  a hobbyist  wit a couple  of fish  - no problem. For discus addict, Aquatics Foods is still  a necessity... sorry.
   
That said I  have a culture  going with  nothing  in the tank  but air and  some fish  food and they are doing very good. I'm also looking at other  media for growing  them in /on but  I've been a little  swamped lately. i will  post about those other  medias  when I  can though!

Take care,  
al

M0oN
12-19-2003, 05:28 AM
I've been told rotten bananna peels work well also, if they're easier to seperate or not I do not know but somthing to try if you're bored.

korbi_doc
12-19-2003, 02:13 PM
:book: :book: "Al", "BORED"??????? :funny: :funny: :funny: :funny: :funny: :funny: :funny: :funny:
Surely you :jester: :jester: